Experimental Biophotonics (FSI-TEB-A)

Academic year 2021/2022
Supervisor: doc. Ing. Daniel Zicha, CSc.  
Supervising institute: ÚFI all courses guaranted by this institute
Teaching language: English
Aims of the course unit:
Objective of the theme is the introduction into the basics and prospective of live cell biology in vitro as observed and studied with techniques of light microscopy.
Learning outcomes and competences:
Acquaintance with the basics of live cell biology in vitro; preparation and cultivation of cell for microscopy observation; application of light microscopies to study cell living in vitro; elements of interpretation of results of biological experiments from physical and biological points of view.
Understanding problems and gaining insight into current trends of biological microscopy.
Acquiring of widened ability to interdisciplinary professional activity.
Prerequisites:
Knowledge of elements of geometric optics.
Course contents:
Basics of cell biology in vitro: structure and functions of the cell; isolation into and maintenance of cells from multicellular organism into in vitro systems; mastering the specialty of preparation of live cells for microscopical observations. Protocols of in vitro investigations of cell behaviour. Interpretation and evaluation of results of microscopical observations of cell behaviour. The role of light microscopy techniques for study of live cells in vitro: principles of light microscopy, fluorescent and confocal microscopy, quantitative phase contrast in holographic microscopy, image processing and analysis.
Teaching methods and criteria:
The course is taught through lectures explaining the basic principles and theory of the discipline. Teaching is suplemented by practical laboratory work.
Assesment methods and criteria linked to learning outcomes:
Student assesment will take into consideration his/her work at laboratory and wiil depend on the result of final discussion on specified themes. Notes and literature is permitted.
Controlled participation in lessons:
Presence in all teaching units is required because of their mutual links. In the case of student absention, tacher will specify alternative requirements.
Type of course unit:
    Lecture  13 × 2 hrs. optionally                  
    Laboratory exercise  13 × 1 hrs. compulsory                  
Course curriculum:
    Lecture Structure and functions of the cell
Cells isolation into in vitro systems
Principles of light microscopy
Mastering the specialty of preparation of live cells for microscopical observations
Protocols of in vitro investigations of cell behaviour.
Fluorescence and confocal microscopy
Quantitative phase contrast in holographic microscopy
Image processing and analysis
Analysis of results of biological experiments
    Laboratory exercise Lecture themes are developed by laboratory demonstrations and laboratory work of students.
Literature - fundamental:
1. B. Alberts et al.: Molecular Biology of the Cell, 5th Ed. Garland Science, 2007.
2. D. Bray: Cell Movements: From Molecules to Motility. Garland Science 2000.
3. D. E. Chandler, R. W. Robertson: Bioimaging. Jones & Bartlett, Boston 2009.
4. A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004.
Literature - electronic:
5. http://library.thinkquest.org/12413/structures.html
6. http://www.biology.arizona.edu/cell_bio/cell_bio.html
7. http://biology.about.com/od/cellbiology/a/cellbiology.htm
8. http://micro.magnet.fsu.edu/
The study programmes with the given course:
Programme Study form Branch Spec. Final classification   Course-unit credits     Obligation     Level     Year     Semester  
N-FIN-P full-time study --- no specialisation -- Col 3 Elective 2 1 W
N-PMO-P full-time study --- no specialisation -- Col 3 Elective 2 1 W