Optical Microscopy and Spectroscopy (FSI-TMK)

Academic year 2024/2025
Supervisor: Ing. Daniel Zicha, CSc.  
Supervising institute: ÚFI all courses guaranted by this institute
Teaching language: Czech
Aims of the course unit:

The goal of the course is to provide students an overview of the principles and methods of light microscopy and spectroscopy, and give them practical experience with relevant devices.
Students will learn about the history and modern techniques and approaches in the field of light microscopy and spectroscopy and have practical experience with relevant devices.
Learning outcomes and competences:
 
Prerequisites:
 
Course contents:

Introduction to light microscopy (historical overview completed by substantial pieces of knowledge of geometric and wave optics, light-microscope optical setup, techniques of light microscopy, and practical knowledge), theoretical description of image formation (wave theory of image formation based on the Abbe theory), fluorescence microscopy, confocal microscopy, interference and holographic microscopy (principle, setup of the device, imaging properties), X-ray computed tomography, spectroscopic methods (Laser-Induced Breakdown, UV, VIS, Raman), polarisation microscopy, computers and cameras in microscopy.


Demonstrations and practical exercises on light microscopy, and spectroscopy and particle spectroscopy are carried out in laboratories.


Teaching methods and criteria:
 
Assesment methods and criteria linked to learning outcomes:
 
Controlled participation in lessons:
 
Type of course unit:
    Lecture  13 × 2 hrs. optionally                  
    Laboratory exercise  13 × 1 hrs. compulsory                  
Course curriculum:
    Lecture

Introduction to light microscopy
Theory of imaging in microscopy
Fluorescence microscopy
Confocal and 2-photon microscopy
Interference and holographic microscopy
4G in holographic interferometry
X-ray computed tomography
Interaction of light with matter
Laser-induced breakdown spectroscopy
Optical and Raman spectroscopy
Computers and cameras in microscopy
Polarization microscopy
    Laboratory exercise

Demonstrations and practical exercises in laboratories follow the lectures.
Literature - fundamental:
1. D. B. Murphy, M.W. Davidson: Fundamentals of light microscopy and electronic imaging. Wiley‐Blackwell 2012.
2. A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004.
3. H. Kuzmany: Solid-state spectroscopy. Springer, 2009.
4. H. Friedrich: Scattering Theory. Springer, Heidelberg, New York, Dordrecht, London 2013.
5. R. Chmelík: Materiály do praktika předmětu Mikroskopie a spektroskopie. Elektronický studijní text, Brno, 2014.
6. E. Keprt: Teorie optických přístrojů 2, Teorie a konstrukce mikroskopu, SPN, Praha 1966.
7. Internetové zdroje: http://micro.magnet.fsu.edu/primer http://microscopyu.com
Literature - electronic:
1. http://micro.magnet.fsu.edu/primer
The study programmes with the given course:
Programme Study form Branch Spec. Final classification   Course-unit credits     Obligation     Level     Year     Semester  
N-FIN-P full-time study --- no specialisation -- Cr,Ex 5 Compulsory 2 2 W
N-PMO-P full-time study --- no specialisation -- Cr,Ex 5 Compulsory 2 2 W