Academic year 2024/2025 |
Supervisor: | Ing. Daniel Zicha, CSc. | |||
Supervising institute: | ÚFI | |||
Teaching language: | Czech | |||
Aims of the course unit: | ||||
The objective of the theme is the introduction of biophotonics, light microscopy techniques for biology, fiber optics and endoscopes, optical micromanipulation, and polymerization of nanostructures. The theme also includes the basics of cell biology and the application of light microscopy in studies of live cells in vitro, and example applications in neurosciences and cancer research. |
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Learning outcomes and competences: | ||||
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Prerequisites: | ||||
Knowledge of elements of geometric optics. |
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Course contents: | ||||
Basics of biophotonics including principles of light microscopy, fluorescence, confocal microscopy, quantitative phase imaging especially in holographic microscopy, polarisation microscopy, fiber optics and endoscopes, optical micromanipulations, and photopolymerization of nanostructures. Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopy observations. Protocols of in vitro investigations of cell behavior. The role of light microscopy techniques for the study of live cells in vitro: Applications in neurosciences and cancer research. Demonstrations and practical sections in the laboratory. |
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Teaching methods and criteria: | ||||
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Assesment methods and criteria linked to learning outcomes: | ||||
Student assessment will take into consideration his/her work at the laboratory and will depend on his/her presentation of selected relevant scientific publication during a colloquium organized at the end. |
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Controlled participation in lessons: | ||||
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Type of course unit: | ||||
Lecture | 13 × 1 hrs. | optionally | ||
Laboratory exercise | 13 × 1 hrs. | compulsory | ||
Course curriculum: | ||||
Lecture | Basic principles of light microscopy for biology |
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Laboratory exercise | Tissue culture and preparation of cells |
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Literature - fundamental: | ||||
1. B. Alberts et al.: Molecular Biology of the Cell, 5th Ed. Garland Science, 2007. | ||||
2. D. Bray: Cell Movements: From Molecules to Motility. Garland Science 2000. | ||||
3. D. E. Chandler, R. W. Robertson: Bioimaging. Jones & Bartlett, Boston 2009. | ||||
4. A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004. | ||||
Literature - recommended: | ||||
5. http://library.thinkquest.org/12413/structures.html | ||||
6. http://www.biology.arizona.edu/cell_bio/cell_bio.html | ||||
7. http://biology.about.com/od/cellbiology/a/cellbiology.htm | ||||
8. http://micro.magnet.fsu.edu/ |
The study programmes with the given course: | |||||||||
Programme | Study form | Branch | Spec. | Final classification | Course-unit credits | Obligation | Level | Year | Semester |
B-FIN-P | full-time study | --- no specialisation | -- | Cr | 2 | Compulsory-optional | 1 | 3 | W |
Faculty of Mechanical Engineering
Brno University of Technology
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Czech Republic
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