Advanced Light Microscopy - Imaging Theory (FSI-9MIA)

Academic year 2023/2024
Supervisor: prof. RNDr. Radim Chmelík, Ph.D.  
Supervising institute: ÚFI all courses guaranted by this institute
Teaching language: Czech or English
Aims of the course unit:
Understanding of principles of 3D light microscopy and microscopic imaging.
Acquiring of methods for fundamental 3D imaging characteristics calculation.
Learning outcomes and competences:
Knowledge of fundamentals of theoretical description of microscope imaging of 3D objects of their relation to experiment and competence to apply them in practice.
Prerequisites:
Wave and Fourier optics, geometrical optics, fundamentals of light microscopy.
Course contents:
The course explains principles of light microscopy of three-dimensional objects in terms of wave optics. Image properties are described using the formalism of 3D pupil and transfer functions. Properties of advanced microscopic techniques for 3D imaging are explained, especially those of confocal microscopy and coherence-controlled holographic microscopy.
Teaching methods and criteria:
The course is taught through lectures explaining the basic principles and theory of the discipline. The lecture is completed by advanced optical demonstrations and experiments with light microscopes.
Assesment methods and criteria linked to learning outcomes:
Examination is oral. Detailed knowledge of explained matter is proved. Examined student can use a literature.
Controlled participation in lessons:
Presence at lectures is not obligatory, but recommended.
Type of course unit:
    Lecture  10 × 2 hrs. optionally                  
Course curriculum:
    Lecture 1. Important knowledge in the field of light microscopy and wave optics
2. Light wave interaction with an object
3. Imaging from the point of view of wave optics
4. Imaging by confocal micorscope
5. Imaging by interference and holographic microscope
6. Imaging by fluorescence microscope
7. Imaging in bright field
8. Another specific cases of imaging
Literature - fundamental:
1. Radim Chmelík.: Trojrozměrné zobrazení v mikroskopii. FSI VUT v Brně, Brno 2003
2. Min Gu: Principles of Three-Dimensional Imaging in Confocal Microscopes
3. Tony Wilson: Confocal Microscopy. Academic Press, London 1990
4. Radim Chmelik et al.: The Role of Coherence in Image Formation in Holographic Microscopy, Progress in Optics 59 (2014) 267-335.
Literature - recommended:
1. M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999
2. J. W. Goodman: Introduction to Fourier Optics. 2nd ed.. McGraw-Hill, New York 1996
The study programmes with the given course:
Programme Study form Branch Spec. Final classification   Course-unit credits     Obligation     Level     Year     Semester  
D-FIN-P full-time study --- -- DrEx 0 Recommended course 3 1 S
D-FIN-K combined study --- -- DrEx 0 Recommended course 3 1 S