prof. RNDr. Radim Chmelík, Ph.D.
E-mail:   chmelik@fme.vutbr.cz  Dept.:   Institute of Physical Engineering Dept. of Optics and Precise Mechanics Position:   Head of Department Room:   A2/209 Dept.:   Institute of Physical Engineering Dept. of Optics and Precise Mechanics Position:   Professor Room:   A2/209

Supervised courses:

• Advanced Light Microscopy - Imaging Theory (9MIA)
• Advanced Light Microscopy - Imaging Theory (DS142)
• Concepts of Biofotonics (9VKB)
• Design of Instruments and Optomechanics I (TK1)
• Diploma Project I (N-PMO) (TOP)
• Diploma Project (N-PMO) (TPP)
• Diploma Seminar I (N-PMO) (TDS)
• Diploma Seminar II (N-PMO) (TSP)
• Fourier Methods in Optics (TFO)
• Friday CEITEC BUT seminar (DS446)
• Friday CEITEC BUT seminar (DS446)
• Fundaments of Optics (TZO)
• Fundaments of Optics (TZO-K)
• Light Microscopy (9MIK)
• Light Microscopy (DS135A)
• Physiological Optics (TF0)
• Specialised Seminar (N-PMO) (TSS)
• Wave Optics (TAO)

Publications:

• GÁL, B.; VESELÝ, M.; ČOLLÁKOVÁ, J.; NEKULOVÁ, M.; JŮZOVÁ, V.; CHMELÍK, R.; VESELÝ, P.:
  Distinctive behaviour of live biopsy-derived carcinoma cells unveiled using coherence-controlled holographic microscopy, PLOS
  journal article in Web of Science
• ŠTRBKOVÁ, L.; ZICHA, D.; VESELÝ, P.; CHMELÍK, R.:
  Automated classification of cell morphology by coherence-controlled holographic microscopy, SPIE
  journal article in Web of Science
• BABOCKÝ, J.; KŘÍŽOVÁ, A.; ŠTRBKOVÁ, L.; KEJÍK, L.; LIGMAJER, F.; HRTOŇ, M.; DVOŘÁK, P.; TÝČ, M.; ČOLLÁKOVÁ, J.; KŘÁPEK, V.; KALOUSEK, R.; CHMELÍK, R.; ŠIKOLA, T.:
  Quantitative 3D phase imaging of plasmonic metasurfaces
  journal article in Web of Science
• ŠTRBKOVÁ, L.; MANAKHOV, A.; ZAJÍČKOVÁ, L.; STOICA, A.; VESELÝ, P.; CHMELÍK, R.:
  The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy, Elsevier B.V.
  journal article in Web of Science
• PLÖSCHNER, M.; KOLLÁROVÁ, V.; DOSTÁL, Z.; NYLK, J.; BARTON-OWEN, T.; FERRIER, D.; CHMELÍK, R.; DHOLAKIA, K.; ČIŽMÁR, T.:
  Multimode fibre: Light-sheet microscopy at the tip of a needle, Springer Nature
  journal article in Web of Science
• ČOLLÁKOVÁ, J.; KŘÍŽOVÁ, A.; KOLLÁROVÁ, V.; DOSTÁL, Z.; SLABÁ, M.; VESELÝ, P.; CHMELÍK, R.:
  Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion., Journal of Biomedical Optics SPIE
  journal article in Web of Science
• KŘÍŽOVÁ, A.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.; KVASNICA, L.; UHLÍŘOVÁ, H.; ZIKMUND, T.; VESELÝ, P.; CHMELÍK, R.:
  Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior
  journal article in Web of Science
• KOLLÁROVÁ, V.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.; VESELÝ, P.; CHMELÍK, R.:
  Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope, SPIE
  journal article in Web of Science
• DOSTÁL, Z.; SLABÝ, T.; KVASNICA, L.; LOŠŤÁK, M.; KŘÍŽOVÁ, A.; CHMELÍK, R.:
  Automated alignment method for coherence-controlled holographic microscope, SPIE
  journal article in Web of Science
• BALVAN, J.; KŘÍŽOVÁ, A.; GUMULEC, J.; RAUDENSKÁ, M.; SLÁDEK, Z.; SEDLÁČKOVÁ, M.; BABULA, P.; SZTALMACHOVÁ, M.; KIZEK, R.; CHMELÍK, R.; MASAŘÍK, M.:
  Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis, PLOS
  journal article in Web of Science
• KOLLÁROVÁ, V.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.; SLABÝ, T.; VESELÝ, P.; CHMELÍK, R.:
  Quantitative phase imaging through scattering media,
  Quantitative Phase Imaging, pp.93360T-1-93360T-8, ISBN 978-1-62841-426-4, (2015), SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
  conference paper
  akce: SPIE Photonics West 2015, San Francisco, 07.02.2015-12.02.2015
• TRUNEC, M.; MACA, K.; CHMELÍK, R.:
  Polycrystalline alumina ceramics doped with nanoparticles for increased transparency, Elsevier Science Ltd.
  journal article in Web of Science
• ZIKMUND, T.; KVASNICA, L.; TÝČ, M.; KŘÍŽOVÁ, A.; ČOLLÁKOVÁ, J.; CHMELÍK, R.:
  Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy
  journal article in Web of Science
• CHMELÍK, R.; SLABÁ, M.; KOLLÁROVÁ, V.; SLABÝ, T.; LOŠŤÁK, M.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.:
  The Role of Coherence in Image Formation in Holographic Microscopy, Elsevier Academic Press
  journal article in Web of Science
• LOŠŤÁK, M.; CHMELÍK, R.; SLABÁ, M.; SLABÝ, T.:
  Coherence-controlled holographic microscopy in diffuse media, OSA
  journal article in Web of Science
• TÝC, M.; KVASNICA, L.; SLABÁ, M.; CHMELÍK, R.:
  Numerical refocusing in digital holographic microscopy with extended-sources illumination
  journal article in Web of Science
• SLABÝ, T.; KOLMAN, P.; DOSTÁL, Z.; ANTOŠ, M.; LOŠŤÁK, M.; CHMELÍK, R.:
  Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope
  journal article in Web of Science
• BOUCHAL, P.; KAPITÁN, J.; CHMELÍK, R.; BOUCHAL, Z.:
  Point spread function and two-point resolution in Fresnel incoherent correlation holography, OPTICAL SOC AMER, 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA
  journal article in Web of Science
• KOLMAN, P.; CHMELÍK, R.:
  Coherence-controlled holographic microscope,
  OPTICS EXPRESS, Vol.18, (2010), No.21, pp.21990-22003, ISSN 1094-4087, Optical Society of America
  journal article - other

List of publications at Portal BUT

Abstracts of most important papers:

• CHMELÍK, R.; SLABÁ, M.; KOLLÁROVÁ, V.; SLABÝ, T.; LOŠŤÁK, M.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.:
  The Role of Coherence in Image Formation in Holographic Microscopy, Elsevier Academic Press
  journal article in Web of Science
  Off-axis digital holographic microscopes (DHM) working with incoherent light have been designed and constructed. Their imaging properties can be changed by variation of the coherence of light. This spans from emulation of classic coherent-light DHM allowing for numerical focusing to incoherent-light DHM characterized by high-quality imaging, no coherence noise, halved limit of lateral resolution, and by coherence-gating effect making imaging in turbid media and optical sectioning possible. We describe theoretically the imaging process of a holographic microscope (HM) and how it is influenced by the coherence of illumination. The 3D coherent transfer function (CTF) reveals the dependence of a spatial frequency passband on the coherence properties of a source. Reduction of coherence leads to the passband broadening i.e. to the resolution enhancement. This effect is obvious also from the form of 3D point spread functions, which allows us to characterize imaging by 3D convolution. Imaging and numerical focusing of planar objects are described by 2D CTF derived from 3D CTF for various defocusing. Results for 2D objects are presented also in a simplified approximate form, which gives deeper insight into the fundaments of imaging. In this approximation, the image formation in a turbid medium by coherence gating is elucidated. In addition, it is shown that the mutual lateral shift of the object and reference beams amplifies higher spatial frequencies of a defocused object and allows an object in a turbid medium to be imaged by diffuse (non-ballistic) light. Important theoretical results are verified experimentally.
• SLABÝ, T.; KOLMAN, P.; DOSTÁL, Z.; ANTOŠ, M.; LOŠŤÁK, M.; CHMELÍK, R.:
  Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope
  journal article in Web of Science
  Coherence-controlled holographic microscope (CCHM) combines off-axis holography and an achromatic grating interferometer allowing for the use of light sources of arbitrary degree of temporal and spatial coherence. This results in coherence gating and strong suppression of coherent noise and parasitic interferences enabling CCHM to reach high phase measurement accuracy and imaging quality. The achievable lateral resolution reaches performance of conventional widefield microscopes, which allows resolving up to twice smaller details when compared to typical off-axis setups. Imaging characteristics can be controlled arbitrarily by coherence between two extremes: fully coherent holography and confocal-like incoherent holography. The basic setup parameters are derived and described in detail and experimental validations of imaging characteristics are demonstrated.
• BOUCHAL, P.; KAPITÁN, J.; CHMELÍK, R.; BOUCHAL, Z.:
  Point spread function and two-point resolution in Fresnel incoherent correlation holography, OPTICAL SOC AMER, 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA
  journal article in Web of Science
  Fresnel Incoherent Correlation Holography (FINCH) allows digital reconstruction of incoherently illuminated objects from intensity records acquired by a Spatial Light Modulator (SLM). The article presents wave optics model of FINCH, which allows analytical calculation of the Point Spread Function (PSF) for both the optical and digital part of imaging and takes into account Gaussian aperture for a spatial bounding of light waves. The 3D PSF is used to determine diffraction limits of the lateral and longitudinal size of a point image created in the FINCH set-up. Lateral and longitudinal resolution is investigated both theoretically and experimentally using quantitative measures introduced for two-point imaging. Dependence of the resolving power on the system parameters is studied and optimal geometry of the set-up is designed with regard to the best lateral and longitudinal resolution. Theoretical results are confirmed by experiments in which the light emitting diode (LED) is used as a spatially incoherent source to create object holograms using the SLM.
• KOLMAN, P.; CHMELÍK, R.:
  Coherence-controlled holographic microscope,
  OPTICS EXPRESS, Vol.18, (2010), No.21, pp.21990-22003, ISSN 1094-4087, Optical Society of America
  journal article - other
  Transmitted-light coherence-controlled holographic microscope (CCHM) based on an off-axis achromatic interferometer allows us to use light sources of arbitrary degree of temporal and spatial coherence. Besides the conventional DHM modes such as quantitative phase contrast imaging and numerical 3D holographic reconstruction it provides high quality (speckle-free) imaging, improved lateral resolution and optical sectioning by coherence gating. Optical setup parameters and their limits for a technical realization are derived and described in detail. To demonstrate the optical sectioning property of the microscope a model sample uncovered and then covered with a diffuser was observed using a low-coherence light source.
• JANEČKOVÁ, H.; VESELÝ, P.; CHMELÍK, R.:
  Proving Tumour Cells by Acute Nutritional/Energy Deprivation as a Survival Threat: A Task for Microscopy, International Institute of Anticancer Research
  journal article in Web of Science
  Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by an acute nutritional and energy deprivation as a survival threat. A phosphate-buffered saline (PBS) survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hours. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal cells. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed an increase in the compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. Studying the PBS survival test with DHM opens a new approach to investigations of the structural integrity of neoplastic cells.