Light Microscopy (FSI-9MIK)

Academic year 2023/2024
Supervisor: prof. RNDr. Radim Chmelík, Ph.D.  
Supervising institute: ÚFI all courses guaranted by this institute
Teaching language: Czech or English
Aims of the course unit:
Understanding of principles of light microscopy and microscopic imaging, knowledge of conventional and advanced microscopic techniques.
Learning outcomes and competences:
Knowledge of fundamentals of light microscopy and competence to apply them in practice.
Prerequisites:
Wave and Fourier optics, geometrical optics.
Course contents:
The course explains principles of light microscopy in terms of geometrical and wave optics. Imaging of extended objects is analysed in details using the formalism of pupil and transfer functions. Fundamentals of advanced microscopis methods are explained as well as the directions of their development (confocal microscopy, fluorescence microscopy, interference microscopy, nonlinear phenomena, Raman scattering, stimulated light emission).
Teaching methods and criteria:
The course is taught through lectures explaining the basic principles and theory of the discipline. The lecture is completed by advanced optical demonstrations and experiments with light microscopes.
Assesment methods and criteria linked to learning outcomes:
Examination is oral. Detailed knowledge of explained matter is proved.
Controlled participation in lessons:
Presence at lectures is not obligatory, but recommended.
Type of course unit:
    Lecture  10 × 2 hrs. optionally                  
Course curriculum:
    Lecture 1. Light as the electromagnetic wave, coherence of light, Hopkins' formula.
2. Fundamentals of geometric optics, optical abberations, elementary parts of the microscope.
3. Historical overview.
4. Wave description of imaging, the Abbe theory, the impulse response.
5. Coherent and optical transfer function.
6. Basic techniques of conventional microscopy, bright and dark field.
7. Phase contrast.
8. Polarized light.
9. Confocal microscopy.
10. Interference microscopy.
11. Fluorescence microscopy, modern techniques of microscopy.
Literature - fundamental:
1. D. B. Murphy: Fundamentals of light microscopy and electronic imaging. Wiley-Liss, Hoboken 2001.
2. Born, M., Wolf, E.: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999
3. A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004.
4. T. Wilson: Confocal Microscopy. Academic Press, London 1990
Literature - recommended:
1. M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999
2. J. W. Goodman: Introduction to Fourier Optics. 2nd ed.. McGraw-Hill, New York 1996
3. E. Keprt: Teorie optických přístrojů 2, Teorie a konstrukce mikroskopu, SPN, Praha 1966.
Literature - additional:
1. L. C. Martin: The Theory of the Microscope.. Blackie, London 1966
Literature - electronic:
1. http://micro.magnet.fsu.edu/primer
The study programmes with the given course:
Programme Study form Branch Spec. Final classification   Course-unit credits     Obligation     Level     Year     Semester  
D-FIN-P full-time study --- -- DrEx 0 Recommended course 3 1 S
D-MAT-P full-time study --- no specialisation -- DrEx 0 Recommended course 3 1 S
D-FIN-K combined study --- -- DrEx 0 Recommended course 3 1 S
D-MAT-K combined study --- no specialisation -- DrEx 0 Recommended course 3 1 S