Concepts of Biofotonics (FSI-9VKB)

Academic year 2023/2024
Supervisor: prof. RNDr. Radim Chmelík, Ph.D.  
Supervising institute: ÚFI all courses guaranted by this institute
Teaching language: Czech
Aims of the course unit:
The aim is to understand the principles of application of photonics in specific areas of biology and medicine.
Learning outcomes and competences:
Knowledge of physical and optical principles of biophotonics, which gives a deeper basis for its practical use.
Prerequisites:
Knowledge of geometric, wave and physical optics.
Course contents:
The course provides an overview of modern techniques using electromagnetic radiation suitable for studying the structure, function and dynamics of biological macro and micro objects and of methods applicable to the detection, diagnosis and treatment of diseases in a non-invasive way.
Teaching methods and criteria:
Lecture.
Assesment methods and criteria linked to learning outcomes:
Doctoral exam in the presented subject extent examining in particular the understanding of the physical nature of biophotonics methods.
Controlled participation in lessons:
Attendance at the lecture is optional.
Type of course unit:
    Lecture  10 × 2 hrs. optionally                  
Course curriculum:
    Lecture 1. Recapitulation of electromagnetic radiation theory.
2. Sources of radiation, lasers.
3. Interaction of radiation with matter, effect of radiation on tissues.
4. Basic methods of optical microscopy.
5. Confocal microscopy, OCT / OCM, holographic microscopy.
6. Fluorescence microscopy, Raman microscopy.
7. Flow cytometry.
8. Optical manipulation with microobjects, application of lasers in medicine.
9. Photodynamic therapy, optical biosensors.
10. Application of micro- and nano-opto-technologies in medicine (retinal replacements).
Literature - fundamental:
1. P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003.
2. J. B. Pawley: “Handbook of Biological Confocal Microscopy”, 2nd Edition, Plenum Press, 1995.
3. Krafft, C: Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs. J Biophotonics 9 (2016) 1362-1375.
The study programmes with the given course:
Programme Study form Branch Spec. Final classification   Course-unit credits     Obligation     Level     Year     Semester  
D-FIN-P full-time study --- -- DrEx 0 Recommended course 3 1 W
D-FIN-K combined study --- -- DrEx 0 Recommended course 3 1 W