Topic of doctoral theses

Key applications for holographic microscopy

Supervisor: prof. RNDr. Radim Chmelík, Ph.D.
Specialist: Ing. Pavel Kolman, Ph.D.
Description:
The fluorescence holographic microscope, unlike a conventional fluorescence microscope, uses two opposed objective lenses and it requires no scanning system. In addition to a well known optical sections (amplitude image), it also provides a phase image revealing information about the optical density of the specimen. Although the microscope is primarily intended for transparent samples capable of emitting radiation (eg fluorescence), it can also be used for holographic imaging of reflective surfaces or for holographic imaging in transmitted light. The task includes the selection of suitable sample types, experiment protocol proposal, design and implementation of accessories for sample preparation, accomodation, fixation and storage in a suitable environment, and sample manipulation during the experiment, experiment control, and testing of the microscope imaging possibilities for different sample types, and publishing results. The aim of the thesis is to find limits of the microscope and to maximize the use of all the imaging capabilities of the microscope and find the most suitable applications for this new imaging technique.
Year Programme Branch Specialisation Mode Language Status
2019/20 D4F-P D-FMI F F cs Approved by branch committee